Mechanisms of resistance to powdery mildew in cucumber

Podosphaera xanthii causes powdery mildew of cucumber, and is associated with significant yield quality losses. Development resistant or tolerant varieties the most effective eco-friendly strategy for management. An important host resistance mechanism based on recognition conserved genes, resulting in durable resistance. To determine mechanisms total RNAs were isolated from cultivar Meltem, line VT18, susceptible local variety Camlica. Expression levels nine genes these plants analysed by Reverse Transcription Polymerase Chain Reaction (RT-PCR). The reactions assessed using microscope observations stained specimens. Serine/threonine (STN7), transcription factor (WRKY22), serine/threonine-protein kinase (D6PKL1), serine/threonine receptor (NFP) induced, as positive regulators defence against mildew. Polygalacturonase Inhibitor (PGIP) did not express after P. inoculation Camlica, susceptibility. After inoculation, callose synthase (CALLOSE) cinnamyl alcohol dehydrogenase (CAD) gene expression increased but Hypersensitive (HR) ROS formation only linked VT18. Powdery development was less Meltem than indicating that cell wall thickening HR play separate roles to this disease.